刺五加组培快繁的研究

Tissue Culture and Rapid Propagation of Acanthopanax senticosus

以刺五加腋芽为外植体,通过比较不同的基本培养基(WPM、MS、1/2MS、White)和植物生长调节物质(6-BA、NAA、IAA、IBA)的组合对腋芽诱导、增殖及生根的影响,来建立一套高效的离体芽再生体系。试验结果表明,最佳芽再生培养基为WPM+6-BA1.0mg.L-1+NAA0.1mg.L-1,芽萌发率可达90%;最佳芽增殖培养基为WPM+6-BA0.5mg.L-1+NAA0.05mg.L-1,增殖倍数为4.8;最佳生根培养基为White+IBA0.5mg.L-1+IAA1.5mg.L-1,生根率可达85.2%。

Using axillary bud explants, an efficient plant regeneration system via organogenesis was established for Acanthopanax senticosus. The results showed that the optimum regeneration medium was WPM ＋ 6-BA 1.0 mg · L^-1 ＋ NAA 0.1 mg · L^-1, germination rate was 90% ; the propagating medium was WPM ＋ 6-BA 0.5 mg · L^-1 ＋ NAA 0.05 mg · L^-1, propagating times was 4.8 ; the rooting medium was White ＋ IBA 0.5 mg · L^-1 ＋ IAA 1.5 mg · L^-1 ,the rooting rate was 85.2%.