灯盏花花药培养初报

Preliminary Study on Anther Culture of Erigeron breviscapus

对灯盏花花药培养诱导单倍体植株进行了研究。结果显示:灯盏花花药愈伤组织培养以附加60g.L-1蔗糖较好,B5和MS培养基相比较,MS培养基较适宜,在MS+NAA1.0mg.L-1+BA0.5mg.L-1+蔗糖60g.L-1的培养基中,花药愈伤组织诱导率可达36.03%。将愈伤组织转移到MS+6-BA1.0mg.L-1中继代增殖后,经芽苗分化、生根后可得到完整植株。再生植株根尖细胞经细胞学鉴定存在单倍体。

The preliminary study was carried out on the anther culture of Erigeron breviscapus. The results showed that during callus induction, 60 g · L^-1sucrose was suitable ; MS medium was better than B5 ; the highest induction efficiency 36.03% was achieved on the medium of MS ＋ NAA 1.0 mg · L^-1 ＋ BA 0.5 mg · L^-1 ＋ su- crose 60 g · L^-1 calli were transferred on medium MS ＋ 6-BA 1.0 mg · L^-1to proliferation. After differentiating and taking roots, plantlets were obtained. The chromosome numbers from root-tip cells of regeneration plant was nine. It showed that the regeneration plant was haploid.