Aurora-A/STK15在上皮性卵巢癌组织中的表达及其与预后的关系

Expression of Aurora-A/STK15 in epithelial ovarian cancer and its prognostic significance

目的探讨Aurora-A/STK15在上皮性卵巢癌组织中的表达变化及其与预后的关系。方法应用显微切割法从石蜡切片上提取总RNA,实时定量PCR和免疫组化方法检测80例原发性卵巢上皮性癌和29例正常卵巢组织中Aurora-A/STK15mRNA及蛋白水平的表达,分析其作为卵巢癌标志物的可行性以及与预后的相关性。结果上皮性卵巢癌组织中Aurora-A/STK15mRNA的表达明显高于正常卵巢组织[(60.5±74.2)对(2.19±1.4),P<0.01],但mRNA的表达水平与卵巢癌的分期和分级以及生存时间无关,P>0.05;上皮性卵巢癌Aurora-A/STK15蛋白的阳性表达率也明显高于正常卵巢组织(87.3%对6.9%,P<0.05),蛋白表达水平随病理分级和手术分期增加而增加,与病理分级密切相关(P<0.05),而与手术分期的相关性无统计学意义(P>0.05);在行理想肿瘤细胞减灭术并行术后辅助泰素化疗的病例组,Aurora-A/STK15蛋白高表达预后好,总的生存时间较低表达病例生存时间长(P<0.05);在除泰素以外的铂类药物化疗组,Aurora-A/STK15蛋白高表达则与不良预后相关,高表达者总的生存时间短(P<0.05)。结论Aurora-A/STK15在上皮性卵巢癌中存在异常表达,且与卵巢癌的分化程度以及手术联合辅助化疗的预后密切相关,有望成为卵巢癌个体化治疗疗效的预测因子。

Objective To investigate the expression of Aurora - A/STK15 and its prognostic significance in epithelial ovarian cancer. Methods Total RNA and protein were extract from formalin- fixed and paraffin embedded tissues of 80 primary epithelial ovarian cancer and 29 normal ovaries. The expression of Aurora- A/STK15 mRNA and protein were detected by quantitative real time polymerase chain reaction （Q- RT - PCR） and immunohisto-chemistry, respectively. Results Expression of Aurora - A/STK15 mRNA in ovarian cancer was significantly higher than that in the controls [（60.5±74. 2） vs. （2.19±1.4）, P〈0. 01]. However, it was not related to differentiation grade, FIGO stage or survival time. Expression of Aurora - A/STK15 protein was also significantly higher in ovarian cancer than in the controls （P〈0.05）. It was significantly related to differentiation grade （P〈0. 05） but not to FIGO stage （P〉0.05）. In the patients undergoing optimal debulking operation and adjuvant chemotherapy with Taxon, the overexpression of Aurora - A/STK15 protein was associated with longer overall survival （P〈 0. 05）. In addition, high expression of Aurora - A/STK15 was associated with poor prognosis in the patients undergoing platinum based chemotherapy without Taxon （P〈0.05）. Conclusions The overexpression of Aurora - A/ STK15 in epithelial ovarian cancer associates with differentiation grade and response after optimal debulking and adjuvant chemotherapy. It might become one of the prognostic markers for the individualized therapy of ovarian cancer.