一种敏感的羧化胶乳阴道毛滴虫凝集试验(附486份试验报告)

A Sensitive Carboxylated Latex Agglutination Test for Detecting Trichmonos Vaginalis (A report of 486 cases)

本文应用两株混合单克隆抗体，共价交联到羧化的聚苯乙烯微粒上。用本胶乳检测486份阴道分泌物标本，并与温片镜检作双盲法对照，乳凝集试验比镜检法检出率高，分别为31．8％、21．8％，P＜0．05，两法有显著性差异。同时，比较了羧化胶乳与物理吸附胶乳的稳定性。显示出羧化胶乳有良好的稳定性。检测方法简便，3～5分钟可出结果。

A latex agglutination test (LAT) with carboxylated latex linked by covalent bonds with the mixed monoclonal antibodies of two lines was established. 486 samples of vaginal secretion were tested by wet-slide microscopy and the latex agglutination test (double blind test). The detectable rate of LAT (31.8% ) was significantly higher than that of wet-slide microscopy (21.8%,P＜O.05 ). Compared with the physically absorbed latex,the stability of carboxylated latex was satisfactory. Furthermore,the LAT was very simple which could yield results in 3~5 min.