不同海拔高度新生儿染色体畸变观察比较研究

COMPARATIVE STUDY OF NEONATAL CHROMOSOME ABERRATION IN DIFFERENT ALTITUDE

目的：了解高原低氧分压对新生儿染色体畸变的影响；方法：常规培养新生儿脐带血（高原１６例，喀什２０例，西安３０例），制片、分带、计数观察；结果：①高原组新生儿中，发生结构改变的染色体１０例，占６２．５％，明显高于喀什组３０．０％及西安组６．６６％（Ｐ＜０．０１），喀什组又高于西安组（Ｐ＜０．０５）；②高原新生儿染色体计数观察１５９４个，发生畸变８３个，占５．２０７％，明显高于喀什组２．４０１％及西安组１．５３２％（Ｐ＜０．０１）；③高原新生儿染色体结构异常３４个，占２．１３３％，其中内复制６个，双微体１４个，碎片断１０个，着丝粒爆开１个，出现断裂或增加３个，明显高于喀什组０．８５％及西安级０．１３３％（Ｐ＜０．０１）；

Objective:To study the effects of hypoxia on neonatal chromosome aberration at high altitude.Methods:66 samples of neonatal cord-blood,which were selected from high altitude (16 cases),Kashigar(20 cases) and Xi'an(30 cases),were cultured as a matter of routine,counted and observed.Results:①the changes of chromosome structure in all samples were as follows,there was a remarkable changes up to 62.5% in high altitude group,30.0% in Kashigar and 6.66% in xi'an group(P<001 or P<005).②1 594 chromosome were counted in high altitude group,83 of them were aberrated (5.207%)and the rang of aberration>in kashigar(2.40%)>in Xi'an(1.532%)(P<0.01).3.34 chromosome of 1 594 were found a abnormd(2.133%),of them 6 endoreduplications,14 minute bodies,10 fragments,1 centric burst and 3 part deletion or insertions in altitude group and the chages were greater than those in Kashigar(0.85%)and Xi'an(0.133%)(P<0.01).conclusions:The mechanism of aberration of chromosome under high altitude condition could be induced by hypoxia.