新生儿滤纸血斑TSH检测TR-IFMA、IRMA、ELISA的对比研究

A comparison of TRIFMA,IRMA and ELISA in the determination of neonatal TSH concentration in dried whole umbilical blood spots on filter paper

对碘缺乏病病区和非病区新生儿脐带滤纸血斑同时利用三种方法检测ＴＳＨ值，结果表明ＩＲＭＡ值＞ＴＲ－ＩＦＭＡ值＞ＥＬＩＳＡ值，三者相关非常显著，相关系数分别是ＴＲ－ＩＦＭＡ与ＥＬＩＳＡ为０．８３８、ＥＬＩＳＡ与ＩＲＭＡ为０．８３５、ＩＲＭＡ与ＴＲ－ＩＦＭＡ为０．７７７。５ｍＵ／Ｌ以下所占百分率ＥＬＩＳＡ为７６．９％、ＴＲ－ＩＦＭＡ为６４．７％、ＩＲＭＡ为４４．８％。经多年补碘的病区与非病区ＴＳＨ值分布频率基本相似。认为三种方法均可用于碘缺乏病监测，但ＩＲＭＡ值偏高，可根据回归方程ＴＲ－ＩＦＭＡ、ＥＩＡ值进行换算。

n order to investigate the correlation of several methods for TSH detection,we studied neonatal cord blood TSH levels detected with timeresolved immunofluorometric assay (TRIFMA),immunoradiometric assay (IRMA) and enzymelinked immunosorbents assay (ELISA).The neonatal cord blood spots on filter paper were collected from IDD area supplied by iodine for many years and nonIDD area.Results showed that TSH levels (x±S,mU/L) of 296 specimens tested by TRIFMA,IRMA and ELISA methods were 5.10±6.05,7.37±6.62,4.04±3.18,respectively.The proportions of specimens with TSH levels less than 5mU/L were 64.7%(TRIFMA),44.8%(IRMA),76.9%(ELISA),respectively.According to TSH levels,correlations among these three methods were:TRIFMA:ELISAr=0.838;ELISA:IRMA r=0.835;IRMA:TRIFMA r=0.777.From TSH values of one assay,we could calculate TSH values of another assay with the regression equations.The frequency distribution curves of the TSH levels in IDD area and nonIDD area were simillar.Our study indicated that these three methods were of quite good correlation,and all could be used in IDD surveillance,but TSH levels detected by IRMA were higher than those by other two methods.