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重组P_(53)基因表达及其与Fas关系初探 被引量:2

Preliminarily Investigating the Expression of theRecombinant P53 Gene and the Relation between the P53 Gene Expression and Fas Gene Expression
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摘要 为观察重组PxT1-P53在真核细胞中的表达及其与Fas表达的关系,笔者采用磷酸钙—DNA共沉淀法,DEAE—莆聚糖介导的DNA转染法将PxT1-P53转移至PA317细胞内,并用抗P53和抗Fas抗体,经间接免疫荧光法证实转染PxT1-P53的PA317细胞不仅能表达P53蛋白,而且亦能表达Fas抗原。本实验结果表明笔者构建的PxT1-P53确实能在真核细胞内表达,并提示P53可能通过其转录因子活性,诱导Fas等基因表达。 おummary:In order to investigate if the constructed recombinant P53 gene could express in the eukaryotes and the relation between P53 gene and Fas gene expression,the 12.2kb recombinant P53 gene was transfirred into the mouse PA317 cell line by the calcium phosphate-DNA coprecipitation method and DEAE-Dextran transfection method,then,the anti-P53 and anti-Fas were used to analyse the expression of P53 and Fas gene in the PA317 cell by indirect Immunofluorecent labeling technique.The Obtained experimental results confirmed the PA317 cells could express the recombinant P53 and suggested the expressing P53 protein could induce Fas gene and others gene to express by the transcriptional activities of P53 proteir to mediated the apoptosis of the cells with damaged DNA.
出处 《川北医学院学报》 CAS 1998年第2期1-3,5,共4页 Journal of North Sichuan Medical College
基金 四川省教委 省科委 省卫生厅资助
关键词 重组 P53基因 基因表达 癌变 抑癌基因 P53 gene Fas gene Recombinatn Expression
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  • 1林松,吴建中,王忠诚,厉俊华,顾建人.人野生型p53基因增强9L胶质肉瘤细胞的化疗敏感性[J]中国肿瘤生物治疗杂志,1997(03).
  • 2(美)J.萨姆布鲁克(J.Sambrook)等著,金冬雁等.分子克隆实验指南[M]科学出版社,1992.

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