胃癌患者外周血中T细胞亚群变化及临床意义

The Changes of T Cell Subsets in Peripheral Blood of Patients with Gastric Cancer and Its Clinical Significance

目的:探讨胃癌患者外周血T细胞亚群的变化及其对评估胃癌患者机体免疫状态的临床意义。方法:流式细胞术分析40例术前胃癌患者及30名健康人群(对照组)外周血中T细胞亚群(CD3+、CD4+、CD8+T细胞、CD4+/CD8+比值),组间比较采用t检验,检验水准a=0.05。结果:研究组胃癌患者外周血CD3+、CD4+T细胞、CD4+/CD8+比值明显低于健康人群对照组,差异有统计学意义(P值均<0.05),而两组间CD8+T细胞的差异无统计学意义(P>0.05);肿瘤侵及浆膜组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于未侵及浆膜组,差异有统计学意义(P值均<0.05);肿瘤有淋巴结转移组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于肿瘤无淋巴结转移组,差异有统计学意义(P值均<0.05);肿瘤临床分期TNMⅢ、Ⅳ期组CD3+、CD4+T细胞、CD4+/CD8+比值明显低于TNMⅠ、Ⅱ期组,差异有统计学意义(P值均<0.05);肿瘤高中分化组和低分化组间CD3+、CD4+T细胞、CD4+/CD8+比值的差异无统计学意义(P值均>0.05),各不同组间外周血CD8+T细胞的差异无统计学意义(P值均>0.05)。结论:胃癌患者术前外周血T细胞亚群对评估胃癌患者机体免疫状态有一定的临床意义,也为肿瘤的免疫增强治疗提供了理论依据。

Objective Explore the changes of T cell subsets in peripheral blood of patients with gastric cancer and its clinical significance to assess the immune status of patients with gastric cancer. Methods Using flow cytometry to analys 40 cases of preoperative patients with gastric cancer and 30 healthy people（control group） in peripheral blood T cell subsets （CD3＋、CD4＋ T cells, CD4＋/CD8＋ ratio）. Inter-group comparison t test were used, significance level a = 0.05. Results CD3＋, CD4＋ T cells, CD4＋/CD8＋ ratio of gastric cancer patients study group was significantly lower than those of the healthy control group, the difference had statistical significance （All P values 〈 0.05）, and between the two groups of CD8＋T cells, difference had no significant（P 〉 0.05）; Serosal invasion tumor group CD3＋, CD4＋T cells, CD4＋/CD8＋ ratio were significantly lower than those of no serosal invasion group, the difference had statistical significance （All P value 〈 0.05）; tumor with lymph node metastasis group CD3＋、CD4＋