川芎嗪和异博定联合逆转HL60/HT细胞的多药耐药

Reversal of multidrug resistance of HL60/HT leukemia cell line by combination of tetramethylpyrazine and verapamil

观察中药钙通道阻滞剂川芎嗪（TMP）和异博定（VP）对白血病HL60／HT耐药细胞系多药耐药（MDR）的逆转．方法：采用四唑氮蓝（MTT）法作为药物敏感实验，流式细胞仪测量细胞内柔红霉素（DNR）浓度．结果：采用非细胞毒剂量的100mg／LTMP和（或）0．32mg／L及1mg／L的VP与DNR联合应用，均能提高DNR的细胞杀伤作用，其效果比较为VP（1mg／L）＋TMP＞VP（1mg／L），VP（0．32mg／L）＋TMP＞TMP＞VP（0．32mg／L）（P＜0．01），而且VP（1mg／L）的逆转效果与VP（0．32mg／L）＋TMP相当．同时用流式细胞仪测量细胞内DNR浓度发现TMP和（或）VP均能增加HL60／HT细胞内DNR的浓度，效果比较VP（1mg／L）＋TMP＞VP（1mg／L）＞TMP．结论：TMP与VP有协同逆转作用，是一种有效的中药白血病MDR逆转剂．

Aim:To investigate the reversal of multidrug resistance (MDR) of leukemia cell lines HL60/HT by traditional calcium channel blocker tetramethylpyrazine (TMP)and verapamil (VP). Methods:The drug sensitivity test wasperformed with MTT and the concentration of daunorubicin(DNR) in cells was measured by flow cytometer (FCM).Results: The cytotoxicity of DNR to HL60/HT was enhanced by TMP and (or) VP at nontoxic doses, the effectwere VP (1 mg/L) +TMP>VP (1 mg/L), VP (0. 32 mg/L ) +TMP>TMPM 0. 32 mg/L VP, and the effect of VP(0. 32 mg/L) +TMP was similar to that of VP (1 mg/L).Meanwhile TMP and (or ) VP increased the concentration of DNR in cells. And the effects were VP(1 mg/L) +TMP>VP (1 mg/L) >TMP. Conclusion: For the reversaleffect of VP was enhanced by being combined with TMP,TMP may be a safe and effective revertant to MDR.