丙戊酸钠诱导Siha细胞周期阻滞的实验观察

Cell cycle block induced by sodium valproate in Siha cells

目的:研究丙戊酸钠对人宫颈癌Siha细胞增殖和细胞周期的影响。方法:MTT检测生长抑制,流式细胞仪检测细胞周期,蛋白质印迹法检测p21WAF1/CIP1、p-CDK2和cyclin E蛋白。结果:丙戊酸钠以剂量依赖性方式抑制Siha细胞生长IC20和IC50分别为2.0 mmol/L和8.0 mmol/L;丙戊酸钠上调G0/G1期比例由(74.92±0.60)%升高至(84.475±1.11)%,P=0.001,下调S期(13.19±0.70)%降为(7.72±1.40)%,P=0.004和G2/M(11.89±1.30)%降为(7.81±0.31)%期比例,P=0.006。丙戊酸钠上调p21WAF1/CIP1蛋白,下调cyclin E和p-CDK2蛋白。结论:丙戊酸钠上调p21WAF1/CIP1表达和抑制cyclin E表达,使细胞阻滞于G0/G1期,抑制Siha细胞生长。

OBJECTIVE： To explore the effect of sodium val- proate on proliferation and cell cycle in Siha cells. METHODS： Growth inhibition rates were determined by MTT assay. Cell cycles were assessed by flowcytometry,p21WAF1/CIP1, p-CDK2, and cyclin E protein were examined by Western blot. RESULTS： Sodium val- proate inhibited the proliferation of Siha ceils in a dose dependent manner IC20 and IC50 were 2.0 mmol/L and 8.0 mmol/L, signifi- cantly. Siha cells showed a G0/G1 phase block, increased from （74. 92± 0. 60） % to （84.475±1.11）%（P=0.001）, and S and G2/M phase decreased in response to sodium valproate. The per- centage of S stage decreased from （13.19±0.70）% to （7.72± 1.40）%（P=0.004）. The percentage of G2/M stage decreased from （11.89±1.30）% to （7.81±0.31）%（P=0.006）. Sodium valproate up-regulated p21WAF1/CIP1 and down-regulated eyclin E and p-CDK2 protein. CONCLUSION： Sodium valproate may play an an- ti-proliferation role by raising p21WAF1/CIP1 protein, reducing cylinE and inducing G0/G1 phase arrest.