子宫内膜癌组织p16基因甲基化检测及其临床意义的研究

Detection and clinical significance of methylation of p16 in endometrial carcinoma tissues

目的:研究子宫内膜癌(endometrialcarcinoma,EC)组织中p16基因CpG岛甲基化情况及其临床意义。方法:采用甲基化特异性PCR法(methylation specific PCR,MSP)检测38例EC组织、20例相应癌旁正常内膜组织(adjacent normal endometrium,ANE)和22例正常子宫内膜对照组织(normal controls,NC)中p16基因甲基化状态。结果:ANE和NC组织中不存在p16基因全部甲基化,EC及ANE组织p16基因部分甲基化率分别为26.3%(10/38)及30.0%(6/20),EC组织全部甲基化率为31.6%(12/38)。EC、ANE和NC组织p16基因甲基化率(全部甲基化与部分甲基化)分别为57.9%(22/38)、30.0%(6/20)和0,差异有统计学意义,χ2=20.821,P<0.01。p16基因甲基化率与EC临床分期(χ2=4.716,P<0.05)及病理分化(χ2=5.739,P<0.05)密切相关。结论:p16基因甲基化在EC发生和发展过程中可能是一个早期的、逐渐发展的事件,可为EC的早期诊断提供一定的理论基础。

OBJECTIVE： To study the CPG islands meth ylation status and clinical significance of p16 gene in endometri- al carcinoma. METHODS： Methylation status of p16 gene was determined by methylation-specific PCR（MSP） in a series of 3 specimens from 38 cases of endometrial carcinoma （EC）, 20 cases of adjacent normal endometrium （ANE）, and 22 cases of normal controls （NC, 5 of simple hyperplasia, 5 of inflamma tory polyp, and 12 of normal endometrium）. RESULTS： There was no completely methylated of p16 gene in specimens of ANE and NC. The rates of partical methylation of p16 gene were 26.3%（10/38） in NC and 30.0%（6/20） in ANE respec- tively, and 12 of the 38 EC showed completely methylated （31.6%（12/38）3. The total methylation rates of p16 gene in EC, AEC and NC were 57. 9X （22/38）, 30.0% （6/20） and 0, respectively. The dif{erence was statistically signi{icant （x2=20. 821,P〈0.01）. The methylation rate of p16 gene was closely related to the clinical stage （2=4.716,P=0.05） and pathologic differentiation x2 = 5. 739, P〈0.05） of endometrial carcinoma. CONCLUSION： Methylation of p16 gene is an early and developing event in carcinogenesis of endometrial carcinoma.