体外利用MGc80-3胃癌细胞诱导肿瘤杀伤细胞的实验研究

Tumor activated killer cells induced by gastric carcinoma cell line MGc803 in vitro

以健康人外周血单个核细胞作为肿瘤杀伤细胞的前体细胞，ＭＧｃ８０３胃癌细胞作为刺激细胞，在体外进行同种异体淋巴细胞和肿瘤细胞混合培养之后，单独加入重组白介素２和同时加入重组白介素２、抗ＣＤ３单克隆抗体，分别诱导出Ｌ肿瘤杀伤细胞和ＣＤ３肿瘤杀伤细胞．用噻唑蓝比色法在体外检测其对ＭＧｃ８０３胃癌细胞的杀伤活性，并与淋巴因子激活杀伤细胞、ＣＤ３肿瘤杀伤细胞相比较结果显示，ＣＤ３肿瘤杀伤细胞的杀伤活性最高．在倒置显微镜下及透射电镜下观察ＣＤ３肿瘤杀伤细胞对ＭＧｃ８０３胃癌细胞的杀伤过程，发现ＣＤ３肿瘤杀伤细胞首先接触、粘附靶细胞。

Tumor activated killer cells(TAK)were generated in human peripheral blood imononuclear cells by stimulating with human gastric carcinoma cell line MGc803 using allogenetic mixed lymphocyte tumor cell culture method. LTAK cells and CD3TAK cells were induced by MGc803 cells with rIL2, and MGc803 cells with rIL2,and antiCD3Ab,respectively.Testing in vitro cytotoxicity against MGc803 cells in MTT colorimetry CD3TAK cells gave the highest levels of cytotoxity compared with LAK cells and CD3AK cells.In vitro cytolysis process of MGc803 cells mediated by CD3TAK cells were observed using inverted microscopy and transmission electron microscopy.The results showed that CD3TAK cells attached the target and the target cells died by apoptosis and necrosis.