Rb1基因在直、结肠癌中的重排与扩增

Amplifications and rearrangements of Rb_1 gene in colorectal carcinomas

目的：检测14例经临床及病理确诊的直、结肠癌及其癌旁组织标本基因组DNA中Rb1基因结构状态。方法：利用Rb1基因特异性cDNA探针，采用Southern杂交分析方法；结果：4例（4／14）现Rb1重排，其中3例有异常酶切片段的扩增门0～20倍）。Rb1基因重排、扩增的病例其临床恶性度高，预后不好。结论：Rb1基因在直、结肠癌中可能有癌基因样功能，其作用机理可能与其在视网膜母细胞瘤等肿瘤中的不尽相同。

Objective: To detect the structure of Rbl gene in DNA from the patients with colorectal carcino-ma and to study the Possible molecular mechanisms of the me tumorgenesis. Methods: Southern blott techniquewas applied with Rb1 gene cDNA specific probe. Resuns: Four of 14 samples showed Rb1 gene rearrangementsand 3 cases out of these 4 presented amplifications at same emzyme restriction fragments. The clinical prognosisof these cases were worse. Conclusion: Rb1 gene might have 'oncogene-like' action during the tumorogenesis ofcolorectal carcinomas,and the molecular mechanism may be differenct from that in retinoblastomas.