sIL-2R在急性移植物抗宿主病中的临床研究

Effect Of sIL-2R On Acute Graft-Versus-Host Disease

目的探讨细胞因子sIL-2R在异基因造血干细胞移植(allo-HSCT)后急性移植物抗宿主病(aGVHD)发病机理中的作用。方法观察20例Allo-HSCT患者aGVHD的发病情况,移植前后定期采集20例患者的外周血,采用双夹心酶联免疫吸附法(ELISA)检测其细胞因子sIL-2R的浓度。结果①异基因造血干细胞移植后20例患者全部获得造血功能重建,中性粒细胞恢复到0.5×109/L及血小板恢复到20×109/L的中位时间分别为移植后13.5d及18d;②发生aGVHD的患者,sIL-2R浓度较移植前明显升高,sIL-2R浓度在aGVHD阳性组明显高于aGVHD阴性组(P<0.01)。结论①细胞因子sIL-2R在aGVHD的发病中起重要的正向调节作用,检测异基因造血干细胞移植后患者血清的sIL-2R水平有助于预测aGVHD的发生;②sIL-2R与感染无相关性。

Objective To study the effect of cytokine sIL-2R on acute graft-versus-host disease （aGVHD） after allogeneic hemopoietic stem cell transplantation（allo-HSCT）. Methods The aGVHD was observed in 20 patients with AIIo-HSCT. The peripheral blood of 20 patients was collected before and after transplantion, the concentration of sIL-2R was detected by enzyme-linked immunosorbentassay （ELISA）. Results The haematogenesis of 20 patients recovered after the allogeneic hemopoietic stem cell transplantation. The median-time that neutrophilic granulocyte recovered to 0. 5 × 109/L was 13.5 days, the median-time that platelet recovered to 20 × 109/L was 18 days. The concentration of sIL-2R in patients with aGVHD significantly in- creased more than that in patients without aGVHD （ P 〈 0. O1 ）, Conclusions sIL-2R has the up-regulated function in Agvhd. It＇ s valuable to detect the concentration of sIL-2R after transplantation for predicting aGVHD.