GnRH及其受体表达与肝癌细胞增殖和分化的关系

Relationship of gonadotropin releasing hormone and its receptor expression with human hepatocarcinoma cell proliferation and differentiation

探讨促性腺激素释放激素（GnRH）及其受体表达与肝癌病理学分级和肝癌细胞增殖的关系．方法：用常规组织学HE染色确定肝癌的病理学分级；用免疫组织化学SABC技术检测49例肝细胞性肝癌（HCC）中增殖细胞核抗原（PCNA），GnRH及其受体的表达．结果：GnRH及其受体均定位于肝癌细胞的胞膜和胞浆．Ⅰ级～Ⅱ级肝癌组织GnRH的阳性率为84．6％（20／23），显著高于Ⅲ级肝癌47．1％（8／17）的阳性率（P＜0．05）和Ⅳ级肝癌11．1％（1／9）的阳性率（P＜0．01）．Ⅰ级～Ⅱ级肝癌组织GnRH受体的阳性率为91．1％（21／23），明显高于Ⅲ级肝癌41．2％（7／17）的阳性率（P＜0．01）和Ⅳ级肝癌11．1％（1／9）的阳性率（P＜0．01）．GnRH阳性肝癌的PCNA标记指数（20．4±12．1）％低于GnRH阴性肝癌（39．1±25．3）％（P＜0．05）；GnRH受体阳性肝癌的PCNA标记指数（19．2±13．5）％亦明显低于GnRH受体阴性肝癌（40．5±29．1）％（P＜0．01）．结论：GnRH可能参与肝癌细胞的增殖和分化的调节．

Aim:To explore the possible relationship of gonadotropin releasing hormone (GnRH) and its receptor(GnRHR) expression with hepatocarcinoma cell proliferation and differentiation. Methods: Proliferating cell nuclearantigen (PCNA),GnRH and its receptor were detected in 49hepatocellular carcinoma (HCC) by immunohistochemicalSABC technique. The pathological degree of HCC wasdemonstrated by hematoxilin and eosin (HE) staining. Results:GnRH and its receptor had the same distribution pattern in hepatocarcinoma cells. The positive signal was foundmainly on the surface and in the cytoplasm of hepatomacells. Edmondson's grade Ⅰ~Ⅱ HCC had a higher GnRHpositive rate (84. 6%; 20/23) than those of grade Ⅲ HCC(47. 1 %; 8/17) and grade Ⅳ HCC (11. 1 %;1/9),the differences were significant (P<0. 05 and P<0. 01 respectively).In grades Ⅰ~Ⅱ HCC GnRHR positive rate was 91. 1 % (21/23),which was higher than 41. 2% (7/17) in grade Ⅲ HCC(P<0.01) and 11.1 % (1/9) in grade Ⅳ HCC (P<0. 01 ).The PCNA labeling index was (20. 4±12. 1 ) % in GnRHpositive HCC and (39.1±25. 3) % in GnRH negative HCC,the difference was significant (P< 0.05 ); similarly, GnRHRpositive HCC also had a lower PCNA labeling index(19.2±13. 5 ) % than that of GnRHR negative HCC (40. 5±29. 1 ) %(P<0. 01 ). Conclusion: GnRH might be involved in theregulation of human hepatocarcinoma cell proliferation anddifferentiation.