两株肝癌细胞对淋巴结成分粘附与转移力关系的探讨

Metastatic abilities hepatocarcinoma cells assciated with theiradhesion abilities to the substance in lymph nodes

Ｈｃａ－Ｆ（Ｆ）和Ｈｃａ－Ｐ（Ｐ）是从小鼠肝癌中分离出来的只经淋巴道转移的同源而具有不同转移能力的两株克隆细胞系，Ｆ细胞转移能力明显高于Ｐ细胞。经淋巴结冰冻切片粘附的动态观察发现：Ｆ细胞粘附率明显高于Ｐ细胞，且高峰提前２４ｈ，粘附时间亦较长。光镜下早期两株瘤细胞均粘附于网状纤维支架上。在瘤细胞聚集区网状纤维连续性中断甚至完全消失。电镜下见Ｆ细胞绒毛较多，并伸出勾状突起插入细胞外基质中，突起周围见空隙存在，使基质连续性中断。Ｐ细胞虽亦与基质粘附，但突起少而不明显未见插入细胞周围基质现象。该结果提示：对淋巴结内无定形基质粘附的特性及其牢固程度很可能是形成淋巴结转移灶的基础；

Hca-F(F) and Hca-P(P) cell lines isolated from the mouse hepatocarcinoma have different metastatic abilities to lymph node. ln this study,the cells from the two cell lines were used to adhere to lymph node frozen section. The adhesion rate was significantly higher and adhesion peak was 24 hours earlier in F cellswith high metastatic abilitg to lymph node than P cells with low one. At early stage,these two kinds of cells preferred red subcapsular sinus to attach. ln vivo,the metastatic cells attach to reticular fibers and break their continuity,which were proved by the reticular fiber staining of lymph node section. Under electromicroscope, F cells had many pseudopodia which stick out into extracellular matrix (ECM) and destroy their continuity, however P cells had few pseudopodia which attach to ECM ,but not stick into it. Obviously,the adhesion ability to ECM of F cells is stronger than that of P cells. This result suggested that the specificity and solidity of tumor cells adhering to ECM of lymph node may be the foundation of metastasis formation in lymph node.