活血熄风方对局灶性脑缺血大鼠内皮素1和一氧化氮含量的影响

Effects of Huoxue Xifeng Decoction on Endothelin-1 and Nitric Oxide Levels of Focal Cerebral Infarction Rats

目的观察活血熄风方对局灶性脑缺血模型大鼠血液和脑组织中内皮素1(ET-1)和一氧化氮(NO)含量的影响,探讨其脑保护的作用机制。方法雄性Wistar大鼠40只随机分为5组:假手术组、模型组及活血熄风方高、中、低剂量组,每组8只。采用光化学法建立局灶性脑缺血大鼠模型,造模前7d灌胃给药,活血熄风方高、中、低剂量组分别给予活血熄风方20、10、5g/(kg·d),假手术组和模型组给予等体积蒸馏水灌胃,造模后24h分别取血及脑组织,放射免疫法测定模型大鼠血浆及脑组织中ET-1含量,分光光度法测定血清及脑组织中NO的含量。结果活血熄风方能降低模型大鼠血浆及脑组织中ET-1含量,降低脑组织中NO含量,升高血清中NO含量,与模型组比较,差异有统计学意义(P<0.05或P<0.01)。结论活血熄风方对局灶性脑缺血模型大鼠的保护作用可能与其降低血浆及脑组织中ET-1含量,降低脑组织中NO含量,升高血清NO含量,从而调节ET-1/NO比值有关。

Objective To observe the effects of Huoxue Xifeng Decoction （HXD） on endothelin-1 （ET-1） and nitric oxide （NO） levels in the blood and brain tissue of focal cerebral infarction （FCI） rats. Methods Forty male Wistar rats were randomized into 5 groups： sham-operation, FCI model and HXD-high, HXD-middle, HXD-low dose groups, with 8 rats in each group. The model of FCI rats was established by photo-chemistry method, and intragastric administration were continue 7 days before operation.HXD-high, HXD-middle, HXD-Iow dose groups were given HXD 20,10,5g/（kg·d） respectively, and other groups were given the same volume distilled water. Blood and brain tissue samples were gotten 24 h after operation, and radio-immunity method was used to measure ET-1 level, spectrophotometric method was used to measure NO level. Results Compared with FCI model group, HXD decreased ET-1 level in plasma and brain tissue after infarction, and decreased NO level in the brain tissue, increased NO level in serum （P〈0.05 or P〈0.01）. Conclusion HXD has a marked protective effect on FCI rats, which may be related to that HXD can decrease ET-1 levels in the plasma and brain tissue, decrease NO level in the brain tissue, increase NO level in serum and regulate the ratio of ET-1/NO.