刺老苞根皮黄酮类化合物对MC3TE-E1成骨细胞OPG和RANKL mRNA基因表达影响的实验研究

Effect of Aralia echinocaulis Hand. Mazz flavonoids on Expression of OPG and RANKL mRNA in MC3T3-E1 Osteoblasts

目的：探讨刺老苞根皮黄酮类化合物对MC3T3-E1成骨细胞骨保护素（OPG）和核因子κB受体激活因子配体（RANKL）mRNA基因表达的影响。方法：体外培养MC3T3-E1成骨细胞,分别加入含0 mol.L^-1、10^-8mol.L^-1、10^-7mol.L^-1、10^-6mol.L^-1刺老苞根皮黄酮类化合物的培养液,48h及96h后分别提取细胞总mRNA,用RT-PCR方法分析OPG/RANKL的mRNA的表达,进行半定量分析。结果：培养48h后,与对照组比较,刺老苞根皮黄酮类化合物增强了OPG mRNA的表达,有显著性差异（P〈0.05或P〈0.01）,VOPG/VRANKL比值增大;96h后,刺老苞根皮黄酮类化合物明显增强了OPG mR-NA的表达,各浓度组与对照组相比,均有显著性差异（P〈0.05或P〈0.01）,各浓度组VOPG/VRANKL比值增大,有显著性差异（P〈0.05或P〈0.01）。结论：刺老苞根皮黄酮类化合物可能通过增加成骨细胞OPG的表达来抑制破骨细胞的分化和成熟,从而抑制骨吸收,达到治疗骨质疏松症的目的。

Objective： To explore the effect of Aralia echinocaulis Hand. Mazz flavonoids on expression of OPG and RANKL in MC3T3 - E1 osteoblasts. Methods： The different Aralia echinoeanlis Hand. Mazz flavonoids concentrations （ 0 mol.L^-1,1 × 10^-8 mol.L^-1 1 × 10^- 7 mol.L^-1,1 × 10^- 6mol.L^-1 ） were added into the culture medium. Total mRNA was prepared from osteoblasts after 48h and 96h, and mR_NA expression of OPG and RANKL were detected by RT - PCR. Results：The mRNA expression of OPG in MC3T3-E1 osteoblasts significandy increase compared with that of the control group,and the VOPG/VRANKL ration was deceased markedly in the treatment of 1 × 10^-6mol· L^-1 group after 48h.After 96h,the mRNA expression of OPG significantly increased and the VOPG/VRANKL ratio was also decreased in the treated group compared with that of the control group. Conclusion： Aralia echinocaulis Hand. Mazz flavonoids sup- press the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by Aralia echinocaulis Hand. Mazz flavonoids.