HPLC法测定藏药材铁棒锤、榜嘎中酯型生物碱的含量

Determination of ester-alkaloids in Aconitum szechenyianum and naviculare by RP-HPLC

目的：建立RP-HPLC法测西藏不同产地的铁棒锤、榜嘎中酯型生物碱的含量方法。方法：采用高效液相色谱法。色谱柱为Aichrom C18分析柱（4.6mm×150mm,5μm）;柱温：35℃;流动相：甲醇-0.05mol.L-1磷酸二氢钾-醋酸-异丙醇（67：173：4：4）;检测波长：230nm;流速：1.0mL.min-1。结果：乌头碱在0.04816~0.09632μg（r=0.9998）范围内呈线性。乌头碱加样回收率为96.3%,RSD为1.87%（n=6）。结论：方法可控,结果稳定,为乌头类药材含量测定提供一个参考方法。

Objective： To establish determination methods of ester-alkaloids in Aconitum szechenyianum and Aconitum naviculare. Methods：The chromatographic method was carried out on Aichrom C18 column using CH3 OH-0.05mol· L^-1 KH2PO4-CH3 COOH-（CH3 ）2CHOH（67 ： 173：4：4） as a mobile phase. The detection wavelength was set at 230nm. The flow rate was 1.0mL·min^-1. The column temperature was 35℃. Results：The aconitine calibration curves were linear at the ranges of 0.04816 -- 0. 09632μg （ r = 0. 9998 ） The average recoveries of aconitine was 96.3 % with RSD = 1. 87%. Conclusion：This method is simple, reliable and provides a reference standard for the quality control of aconitum alkaloids.