蒙药酒制狼毒定性研究

Study of identify on winous chamaejasme

目的：建立蒙药酒制狼毒定性鉴别标准。方法：采用显微鉴别方法找到酒制狼毒粉末的显微特征;采用薄层色谱方法,确定酒制狼毒薄层色谱鉴别特征;同时进行了浸出物测定和灰分检查测定。结果：酒制狼毒粉末在镜下可观察到淀粉粒、木栓细胞、导管和油滴;薄层色谱中用环己烷-乙酸乙酯-甲酸（10：7：1）系统展开,紫外灯（365nm）下观察;用环己烷-乙酸乙酯-甲酸（10：5：1）系统及环己烷-三氯甲烷-甲醇-甲酸（5：5：1：0.5）系统展开,喷以硫酸-乙醇（1：1）溶液,热风吹数分钟,紫外灯（365nm）下观察,可见到其特征斑点。酒制狼毒灰分在6%以下,乙醇浸出物在2.5%以上。结论：本实验结果可作为蒙药酒制狼毒的定性鉴别标准。

Objective：To establish a system of Mongolian medicinal liquor chamaejasme qualitative criteria to identify. Methods：Micro- identification system to find a wine chamaejasme microscopic characteristics of the powder; TLC method used to determine wine - chamaejasme TLC characteristics; at the same time extract the determination of ash and the determination of inspection. Result：wine - chamaejasme powder can be observed under the starch grains, cork cells,and oil droplets catheter; TLC with cyclohexane- ethyl acetate- formic acid（ 10：7：1 ）system Start ultraviolet light （365nm）were observed; with cyclohcxane- ethyl acetate- formic acid（ 10：5：1 ） and cyclobexane system- chlo- roform - methanol - formic acid（5：5：1：0.5） system started to spray acid - ethanol（1 ： 1） solution,wind and heat for a few minutes of UV light（365nm） under observation,can see the features of its spots, winous ehamaejasme 6% ethanol extract of 2.5%. Conclusion：The results can be used as medicinal liquor Meng-chamaejasme qualitative criteria to identify.