不同抗原修复方法在免疫组化染色的应用比较

Comparison of the effects of different antigen retrieval methods on immunohistochemical staining

目的研究不同抗原修复方法对食管癌中免疫组化染色结果的影响。方法选取Ki67、IL17、CD4和CD8四种分别定位于细胞核、细胞浆与细胞膜的不同抗原做为免疫组化染色的标记,在食管癌连续切片上应用高压抗原修复和微波抗原修复进行比较分析。结果抗原高压修复的染色效果要优于微波修复。一方面,CD4抗原高压修复染色的阳性率要高于微波修复染色的阳性率;另一方面,CD8、Ki-67和IL-17高压修复染色的阳性率与微波修复染色的阳性率无显著性差异。结论在组织不易脱片的情况下,优先考虑使用抗原高压修复;在微波修复与高压修复染色阳性率没有显著性差异的情况下,应该尽可能选择微波修复这种较柔和的方法以避免组织脱片。

Objective To study the effects of different antigen retrieval methods on immunohistochemical staining of esophageal carcinoma tissues. Methods Four different antigens, Ki67, IL17, CD4 and CDs, were respectively located in the nucleus, cytoplasm and cell membrane as markers for immunohistochemical staining. The effects of high-pressure antigen retrieval and microwave antigen retrieval on immunohistochemical staining serial section of esophageal carcinoma were comparably analyzed. Results The high-pressure antigen retrieval is better than the microwave antigen retrieval. On the one hand, the positive rate of stained CD4 by highpressure antigen retrieval was higher than that of microwave antigen retrieval; On the other hand, there were no significant differences in the positive rates of stained CDs, Ki-67 and IL-17 between the high-pressure antigen retrieval and the microwave antigen retrieval. Conclusion High-pressure antigen retrieval was preferred choice when organization is not easy in the case of off-chip. Taking into account that some cancer tissues are unstable under high pressure, the gentler microwave antigen retrieval method would be a better choice when there are no significant differences in the positive rates of stained antigens between the high-pressure antigen retrieval and the microwave antigen retrieval.