摘要
目的探讨非癌鼻咽上皮细胞发展为鼻咽癌(NPC)的过程中,EB病毒在什么阶段进入鼻咽上皮。方法利用广州中山医科大学肿瘤防治中心四会县防癌基地1994年度复查工作及肿瘤医院门诊,收集鼻咽癌及各类“癌前状态”及“癌前病变”患者蜡块450余例,应用改进后的多聚酶链反应(PCR)方法进行EBVDNA酶基因片段扩增检测。结果NPC浸润癌组织EBVDNA酶基因片段扩增阳性率为97.3%(145/149),NPC原位癌4例中,基因阳性者2例,而防癌基地“癌前状态”人群155例中阳性者2例,阳性率为1.3%(2/155)。临床癌前病变组阳性率为零(0/47)。结论EB病毒在非癌鼻咽组织中出现频率十分低,EB病毒可能在鼻咽上皮癌变后才进入鼻咽上皮,提示EB病毒可能不是NPC的病因。
Objective To examine if EB virus enters nasopharyngeal epithelial cells in the course of the tumorigenesis in nasopharyngeal carcinoma (NPC). Methods Based on the annual investigation of Si Hui county, a cancer prevention base of the Sun Yet sen University of Medical Sciences and Tumour Hospital out patient clinic, more than 450 paraffin embedded tissues with NPC, and with pre cancerous lesions in high risk populations were collected for the detection of EBV DNase gene fragment by an optimized PCR method. Results In 145 of the 149(97.3%) cases with invasive NPC, and 2 of 4 cases with in situ NPC, EBV DNase gene was detected. However, in Sihui, Canton, a high risk county for NPC, only 2 of the 155 subjects with nasopharyngeal precancerous state was the gene positive, and none of the 47 cases examined in the out patient department of the Tumor Hospital with precancerous lesions in the nasopharynx was positive. Conclusion EBV is seldomly detected in the non malignant and precancerous lesions in the nasopharynx in high risk populations. It implies that EBV is probably not etiologically involved in the genesis of NPC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1998年第4期251-253,共3页
Chinese Journal of Oncology
基金
国家八五攻关基金
关键词
鼻咽肿瘤
癌前状态
聚合酶链反应
E-B病毒
Nasopharyngeal neoplasms Precancerous conditions Epstein Barr virus Genes, viral Polymerase chain reaction