口腔鳞癌患者外周血、癌灶及颈淋巴结中T亚群细胞表型的研究

PHENOTYPIC CHARACTERISTICS OF TCELL SUBSETS IN PERIPHERAL BLOOD,TUMOR TISSUES AND LYMPH NODES OF PATIENTS WITH HEADAND NECK SQUAMOUS CELL CARCINOMA

本文对３０例口腔鳞癌患者的外周血（ＰＢＬ）、癌灶局部（ＴＩＬ）、颈深上、中、下三群颈淋巴结（ＬＮＬ）的Ｔ亚群细胞水平通过流式细胞仪和免疫组化ＡＢＣ法进行了研究。结果：鳞癌患者ＰＢＬ中ＣＤ３细胞数比健康人低下（Ｐ＜０．０５），尤其是晚期患者。有颈淋巴结转移者ＴＩＬ中ＣＤ８细胞数明显减少。各群ＬＮＬ中Ｔ亚群细胞水平不同；有肿瘤浸润的淋巴结中ＣＤ４、ＣＤ８细胞数都减少（Ｐ＜０．０５）。Ｔ亚群细胞水平在ＰＢＬ、ＴＩＬ、ＬＮＬ三者中并不相同，但又具有相关性，由此我们认为口腔鳞癌患者ＰＢＬ中的总Ｔ细胞水平下降ＴＩＬ中的ＣＤ８细胞水平低下。各群ＬＮＬ中的Ｔ亚群细胞水平有差异。检测外周血中的Ｔ亚群细胞水平可以推测Ｔ亚群细胞在ＴＩＬ和ＬＮＬ中的分布。

This study evaluated T cell subsets in peripheral blood, tumour tissues and cervical lymph nodes of 30 patients with head and neck squamous cancers. The flow cytometry experiment and the avidinbiodinperoxidase method using monoclonal antibodies were employed. The percentage of CD3 cells in PBL was found to be lower in patients than that of control (P<005). The numbers of T cell subsets of 3 groups in LNL were significantly different. The patients with cervical metastases had fewer CD4, CD8 cells in LNL. The T cell subpopulations in PBL. TIL and LNL were different and interrelated. This data indicated that the number of total T cells in PBL is decreased in patients with SCCHN. The number of CD8 cells in TIL is few. The numbers of T cell subsets of the 3 groups in LNL are significantly different. We can know the percentage of T cell subsets in TIL or in LNL by detecting the proportion of T cell subsets in PBL.