摘要
目的研究Rb基因异常与口腔鳞状细胞癌发生及临床病理指标的关系。方法应用地高辛高效引物标记Rb3.6KbDNA探针,结合抗地高辛抗体碱性磷酸酶显色法,对口腔鳞癌病人中Rb基因改变进行了研究。结果在7/28例口腔鳞癌中发现Rb基因结构异常,其中6例出现5.4、4.1及2.3Kb片段缺失,1例出现6.2Kb新片段;Rb缺失与临床病理指标无显著相关性。结论口腔鳞癌中存在Rb基因缺失、重排。
Objective: To study the correlation between abnormality of Rb gene in initiation of oral squamous cell carcinoma (SCC), and to analyze the relationship between deletion of Rb and some clinicopathological parameters.Method: DIGHighPrime Labeled Rb 36Kb DNA probe in combination with antiDIGAP detection system was used in this study.Results: The structural abnormal alterations of Rb gene were found in 7 cases of total samples, in which observed were the deletion of 54, 41 and 23Kb fragment in 6 cases. Rearrangement was detected in one case. Further analyses were carried out to determine whether the deletion of Rb gene correlated with various clinicopathological parameters of the patients. No correlation was found with sex, age, site of primary tumor, level of histopathological differentiation and TNM staging.Conclusion: The abnormalities of Rb gene might be associated with the carcinogenesis of oral SCC.
出处
《口腔颌面外科杂志》
CAS
1998年第2期106-108,共3页
Journal of Oral and Maxillofacial Surgery
关键词
RB基因
口腔鳞状细胞癌
基因缺失
基因重排
Rb gene Oral squamous cell carcinoma Gene deletion Gene rearrangement