3’-大豆苷元磺酸钠在缺血再灌注损伤时对视网膜抗氧化能力及NOS活性的影响

Effects of DSS on anti-oxidation and NOS activity after retinal ischemia/reperfusion injury in rats

目的研究3’-大豆苷元磺酸钠(DSS)在大鼠视网膜缺血再灌注损伤时对视网膜抗氧化能力及一氧化氮合酶(NOS)活性的影响。方法24只SD大鼠按随机数字表法分成4组:对照组、视网膜缺血再灌注模型组、低剂量(1mg/kg)及高剂量(2mg/kg)DSS组。颈总动脉夹闭法制作视网膜缺血再灌注模型,比色法测定血清丙二醛(MDA)、一氧化氮(NO)浓度及NOS、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果与对照组相比,模型组血清MDA浓度升高(P<0.05),NO浓度降低、eNOS活性降低(P<0.01)。与模型组比较,低剂量DSS组MDA浓度降低、SOD活性升高(P<0.05);高剂量DSS组GSH-Px活性升高(P<0.05),tNOS活性升高(P<0.05);DSS低剂量组(P<0.05)及高剂量组(P<0.01)NO浓度与模型组比较均升高,低剂量及高剂量DSS组eNOS活性均升高(P<0.05)。结论DSS可通过提高eNOS、SOD及GSH-Px的活性,从而提高视网膜缺血再灌注损伤时的抗氧化能力及NO的释放,降低组织细胞损伤,保护眼功能。

Objective The goal of this study was to research the effect of 3 ＇ -daidzein sulfonic sodium＇ s （DSS） on anti-oxidation and nitric oxide synthase（NOS） activity following retinal isehemia/reperfusion （RI/R） injury. Methods Twenty- four male SD rats were divided into sham control group,model group of RI/R injury,low-dosage DSS group and high-dosage DSS group randomly. The model of RI/R injury was created by dissociating and clamping the left CCA for 1 hour and then open the CCA for 1 hour in 18 SD rats,and the CCA was not clamped in sham group. 1 mL/kg normal saline solution （NS） was injected via sublingual vein after operation in sham group and model group,and 1 mg/kg and 2 mg/kg DSS was utilized at the same way respectively in low dosage or high dosage. After clamped CCA for 1 hour and reperfused for 1 hour,the vein blood was collected for detection of contents of malondialdehyde （MDA） and NO in serum, the activity of NOS, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） was determined by colorimetric method. The use of animal followed the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission. Results The content of MDA in serum was obviously increased in model group compared with sham group （P 〈 0. 05 ） ,and that in low-dosage DSS was decreased in comparison with model group（ P 〈 0.05 ）. The activity of GSH-Px in high-dosage DSS group was higher than that of model group （ P 〈 0.05 ）. The activity of SOD in low-dosage group was significantly higher than that of model group （ P 〈 0.05）. The content of serum NO in model group was lower than in sham group （P 〈0. 001 ）. The content of serum NO in model group was lower than in low-dosage DSS group （P 〈0. 05） and high-dosage DSS group （P 〈0. 01 ） respectively. The activity of total NOS in low-dosage group was higher than that in model group （ P 〈 0. 05 ） , and the endothelial NOS in two DSS groups showed an increase in comparison with model group（P 〈 0.05）. Conclusion DSS can protect retina against cellular damage in RI/R injury eye through elevating the content of NO,activity of SOD and GSH-Px and increasing anti-oxidation of retina.