玻璃体切割术后兔晶状体纤维细胞凋亡的研究

Experimental research on apoptosis of lens fiber cells after vitrectomy in rabbit

目的探讨玻璃体切割术后兔晶状体纤维细胞的凋亡及意义。方法将30只Dutch Belted兔按随机数字表法分为3组,每组10只。右眼行部分玻璃体切割术,玻璃体腔分别置换平衡盐溶液(BSS)、20%C3F8、硅油。术后1、3、7d,1个月、3个月观察晶状体混浊情况,组织病理学观察晶状体纤维细胞形态;原位末端核苷标记(TUNEL)法检测凋亡,并计算凋亡指数(AI)。对照组为健康兔眼晶状体。结果实验各组术后晶状体不同程度混浊;晶状体上皮细胞(LECs)下的纤维细胞核较对照组增大、变圆。实验组术后1d晶状体纤维细胞凋亡增加,3d、7d达高峰,1个月后凋亡减少。3个月内实验各组AI较对照组明显增高(P<0.05)。结论玻璃体切割术可诱导晶状体纤维细胞凋亡增加,凋亡的增加可能是玻璃体切割术后并发性白内障的致病原因之一。

Objective The microenvironment change after vitrectomy can induce lens opacification. Studies showed this procedure is associated with apoptosis of lens fiber cells. Present study was designed to explore the apoptosis of lens fiber cells after vitrectomy. Methods Thirty Dutch Belted rabbits were randomly divided into 3 groups. The right eyes of rabbits underwent partial vitrectomy. Vitreous was respectively replaced by balance salt solution （BSS） ,20% C3 F8 or silicone oil during the operation. The statue of lens opacity was evaluated under the silt lamp microscope. Two eyes were enucleated in each group at 1 day,3,7 days and lmonth,3 months after surgery for the pathological examination of lens fiber cells under the light microscope. Apoptosis of lens fiber cells was detected by TUNEL and quantified by calculating the apoptotic index（AI） . Two fellow eyes were as the controls. The use of animals followed the ＂ Regulation for the Administration of Affair Concerning Experimental Animals＂ issued by State Science and Technology Commission. Results The posterior subcapsular cortical opacifieation of lens appeared in the 3rd day in C3F8 group, and obvious opaeification was found in 1 month in silicone group. In BSS group, focal cortical opaeification was seen in 1 month after operation. The lens in C3F8 and silicone and BSS groups showed different degrees of opacity. In normal control group,the shape of the lens fiber nuclei under the lens epithelial cells was elongated ; while in Ca F8 , silicone and BSS groups,the lens fiber nuclei at the same location presented a more spherical shape. On the 1 st day after surgery, apoptosis was seen on the lens fiber cells and reached a high level on the 3rd and 7th day in C3 F8 , silicone and BSS groups. The AI in C3 F8, silicone and BSS groups was significantly higher than that of the control group in 3 months （ P 〈 0.05 ）. The AI of silicone oil group was higher than that of BSS group in the 3rd day and both BSS group and C3F8 group in 3 months（P 〈0.05）. Conclusion Vitrectomy induce apoptosis of lens fiber cells,which may be one of the etiological factors for complicated cataract.