花椒对小白鼠子宫平滑肌收缩的影响及其机制的实验研究

Ekperimental Study on Effects of Zanthoxylum Maxim on Contraction of Uterine Smooth Muscle of MUS muscolvs albus and It’ s Mechanism

目的：研究花椒挥发油（EOZM）对离体未孕雌激素预处理小白鼠子宫平滑肌的作用及其可能的机制。方法：分别观察EOZM和维拉帕米（Vet）对催产素（OXT）和氯化钙（CaCl2）引起的子宫收缩的影响、对OXT诱导的依内钙性收缩和依外钙性收缩的影响。结果：EOZM和№均可抑制子宫自律性收缩以及OXT和EaCh引起的子宫收缩而且具有剂量依赖性；EOZM对OXT诱导的依内钙性收缩和依外钙性收缩均有抑制作用；Ver仅对OXT诱导的依内钙性收缩有抑制作用，对OXT诱导的依外钙性收缩则没有影响。结论：EOZM可能主要通过阻断受体调控的钙通道（ROC）和电压依赖的钙通道（VDC），减少外钙内流和内钙释放从而抑制小白鼠子宫平滑肌的收缩。

Objective： To study the effects of EOZM on contraction of uterine srnoothmuscle treated by estrogen of Mus musculus albusand its possible mechanism. Methods： To observe the effects of EoZM and Ver on contraction induced by OXT and CaCl2and on intracellUlar and extracellUlar calCium dependent contraction induccd by OXT respectvely.Results： Both EOZM and Vex COUld inhibit the contraction induced by OXT and CaCl2 and intracellular calcium dependent contraction induced by OXT and the effects are does dependent. EOZM also inhibied extracellular Calcium dependent contraction induced by OXT but Vex not. Conclusions, EOZM inhibits the contraction of uterus mainly by blocking ROC and VDC and consequently decreasing influx Of extracellular calCium and decreasing release of intracellular Calcivm.