产雌孤雌松毛虫赤眼蜂低温贮存的研究

Research on Cold Storage of Thelytoky Line of Trichogramma dendrolimi

在7℃恒温条件下,对用柞蚕卵繁殖的产雌孤雌松毛虫赤眼蜂卵期、幼虫初期、幼虫末期、预蛹期4个虫态进行了30 d及60 d的贮存试验。结果表明,低温贮存后对羽化出蜂数以及寄生、羽化能力均有影响。除卵期贮存未发育外,其他各贮存虫态产雌孤雌品系松毛虫赤眼蜂羽化出蜂数、单头产卵量及羽化率随着贮存时期的延长而下降。贮存30 d,预蛹期的羽化出蜂数最高,为89.00头,幼虫初期的单头产卵量及羽化率最高,分别为85.27粒和84.85%。贮存60 d,各贮存虫态羽化出蜂数、单头产卵量及羽化率差异均不显著。低温贮藏未影响松毛虫赤眼蜂的产雌孤雌的生殖方式,后代没有雄蜂出现。

Egg, early larva, late larva and prepupa periods of thelytoky line of Trichogramma dendrolimi reared on A ntheraea pernyi eggs were researched on cold storage for 30 days and 60 days, at 7℃. The results showed that cold storage had an effect on both emergence number and ability of adult parasitic and emergence of thelytoky line. In addition to egg period, the other storage periods of emergence number, adult parasitic number per female and emergence rate of thelytoky line of Trichogramma dendrolimi were decline as increasing of storage time. For 30 days, the emergence number of prepupa period was maximum, which was 89.00. Both adult parasitic number per female and emergence rate of early larva period were maximum, which were 85.27 and 84.85%, respectively. For 60 days, the differences of each storage period of emergence number, adult parasitic number per female and emergence rate of thelytoky line of T. dendrolimi were not significant. Cold storage did not affect the sex rate of thelytoky line of T. dendrolimi, and all of the offspring were female, there was no male wasp appeared.