铅离子对栉孔扇贝幼贝几种免疫因子的影响

Effect of Pb^(2+) on Immune Factors of Larval Chlanys farreri

测定了栉孔扇贝幼贝在不同浓度Pb2+胁迫下体内酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的活力变化以及过氧化氢(H2O2)含量的变化情况。结果表明,当Pb2+浓度为0.01 mg.L-1时栉孔扇贝幼贝体内ACP和AKP活力明显高于对照组,且差异极显著(P<0.01),随Pb2+浓度的增加,酶活力呈下降趋势,在0.05mg.L-1时酶活力低于对照组;栉孔扇贝幼贝H2O2含量的变化在0.01 mg.L-1和0.02 mg.L-1低浓度Pb2+影响下呈缓慢升高的趋势,但总体变化不明显,而当Pb2+浓度达到0.05 mg.L-1时,H2O2含量明显高于对照组,且差异极显著(P<0.01),随Pb2+浓度的增加,H2O2含量缓慢增加。说明Pb2+能破坏幼贝机体的免疫防御功能,在海洋污染中,Pb2+胁迫是使栉孔扇贝幼苗抗病力减弱、种质退化、并引起大量死亡的一个较为重要的因素。

The effects of different concentrations of plumbum ion （Pb^2＋） on the activity of acid phosphatase （ACP） and alkaline phosphatase （AKP） and quantity of hydrogen peroxide （H2O2） in larval Chlamys farreri were studied in this paper. The results showed that when the concentration of Pb^2＋ was 0.01 mg·L^-1, both the ACP and AKP activity in larval scallop were higher than the control group, but at the level of 0.05 mg·L^-1, their activities were lower than the control group. With the increasing Pb^2＋ concentration in the range of 0.01 mg·L^-1 to 0.02 mg·L^-1, the quantity of H2O2 increased slowly. When the concentration of Pb^2＋ reached at 0.05 mg·L^-1, the quantity of H2O2 was higher than the control group （P〈0.01） and increased slowly afterward. The results showed that the immune system of larval scallop could be destroyed by Pb^2＋ contamination.