宫颈糜烂患者单纯疱疹病毒感染的检测

Detection and Typing of Herpes Simplex Virus in Cervical Specimens from Patients with Cervicitis

目的了解宫颈糜烂患者单纯疱疹病毒(HSV)感染状况。方法用抗原捕获聚合酶链反应(AC-PCR)对450份妇女宫颈棉拭标本进行HSV检测与分型。结果HSV的检出率为25.5%(115/450),其中HSV-1和HSV-2的检出率分别为6.4%(29/450)和19.1%(86/450)。宫颈糜烂组和正常对照组的检出率分别为30.2%(100/331)和12.6%(15/119),两组间检出率差异有统计学意义(χ2=14.26,P<0.005)。随宫颈糜烂程度的增加,HSV感染率增加。结论HSV感染与宫颈糜烂的发生密切相关,以HSV-2感染为主。

Objective To understand the proportion of herpes simplex virus （HSV） in cervical specimens from patients with cervicitis. Methods HSV in cervical specimens from 450 patients with cervicitis was detected and typed by AC - PCR. Results AC- PCR was used to detect HSV DNA in 450 swabs from the female cervical lesions. 115 cases were found positive, in which there were 29 cases of HSV - 1 （6.4 % ） and 86 cases of HSV - 2 （ 19.1% ）. One hundred （30.2 % ） of the 331 patients with cervicitis and fifteen （12.6%） of the 119 controls were positive for HSV, there were statistically significant differences in detection rate between cervicitis group and control group （χ^2 = 14.26, P〈 0. 005）. The infection rate of HSV increased with the increment of cervicitis degree. Conclusions The results indicate that HSV infection is closely related to cervicitis, moreover, HSV- 2 is a common cause of cervicitis.