摘要
利用3种方法DPPH、ABTS和FRAP分析两种苦苣苔科植物卷丝苣苔和勐醒芒毛苣苔提取物总抗氧化作用。在4种提取物中,卷丝苣苔甲醇提取物对DPPH自由基的清除能力(IC50=4.92μg/mL)比阳性对照BHT作用强(IC50=18.79μg/mL);卷丝苣苔甲醇提取物对ABTS自由基的清除能力(IC50=11.10μg/mL)比BHT(IC50=6.04μg/mL)略低;卷丝苣苔甲醇提取物还原Fe3+的能力(FRAP=2403.77±38.05μmolTE/g)比BHT(FRAP=1748.49±3.46μmolTE/g)高。在4种提取物中,卷丝苣苔甲醇提取物抗氧化能力最高。
The total antioxidant effect of extracts from Corallodiscus kingianus and Aeschynanthus mengxingensis was assayed by three methods,namely, DPPH radical scavenging, ABTS radical scavenging and ferric reducing antioxidant power (FRAP) assay. In four extracts from two plants,the methanol extract from C. kingianus showed a higher active of DPPH radical scavenging activity with IC50 value of 4.92 μg/mL than that of positive control BHT with IC50 value of 18.79 μg/mL. The methanol extract from C. kingianus showed a less active of ABTS radical scavenging activity with IC50 value of 11.10 μg/mL than that of BHT with IC50 value of 6.04 μg/mL. The methanol extract from C. kingianus exhibited higher active of ferric reducing antioxidant powers with FRAP value of (2403.77 ± 38.05 ) μmol TE/g than that of BHT with FRAP value of (1748.49 ± 3.46) μmol TE/g. The methanol extract from C. kingianus showed the highest antioxidant activity among the four extracts of C. kingianus and A. mengxingensis.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第3期470-472,共3页
Natural Product Research and Development
基金
河南省科技厅基础前沿计划(0723004502)
河南省教育厅基础研究计划(2008A360002)
关键词
卷丝苣苔
勐醒芒毛苣苔
抗氧化活性
DPPH
ABTS
FRAP
Corallodiscus kingianus (Craib) Burtt
Aeschynanthus mengxingensis W. T. Wang
antioxidant activity
DP- PH
ABTS
FRAP