卷丝苣苔和勐醒芒毛苣苔抗氧化活性研究

Antioxidant activity of Corallodiscus kingianus and Aeschynanthus mengxingensis

利用3种方法DPPH、ABTS和FRAP分析两种苦苣苔科植物卷丝苣苔和勐醒芒毛苣苔提取物总抗氧化作用。在4种提取物中,卷丝苣苔甲醇提取物对DPPH自由基的清除能力(IC50=4.92μg/mL)比阳性对照BHT作用强(IC50=18.79μg/mL);卷丝苣苔甲醇提取物对ABTS自由基的清除能力(IC50=11.10μg/mL)比BHT(IC50=6.04μg/mL)略低;卷丝苣苔甲醇提取物还原Fe3+的能力(FRAP=2403.77±38.05μmolTE/g)比BHT(FRAP=1748.49±3.46μmolTE/g)高。在4种提取物中,卷丝苣苔甲醇提取物抗氧化能力最高。

The total antioxidant effect of extracts from Corallodiscus kingianus and Aeschynanthus mengxingensis was assayed by three methods,namely, DPPH radical scavenging, ABTS radical scavenging and ferric reducing antioxidant power （FRAP） assay. In four extracts from two plants,the methanol extract from C. kingianus showed a higher active of DPPH radical scavenging activity with IC50 value of 4.92 μg/mL than that of positive control BHT with IC50 value of 18.79 μg/mL. The methanol extract from C. kingianus showed a less active of ABTS radical scavenging activity with IC50 value of 11.10 μg/mL than that of BHT with IC50 value of 6.04 μg/mL. The methanol extract from C. kingianus exhibited higher active of ferric reducing antioxidant powers with FRAP value of （2403.77 ± 38.05 ） μmol TE/g than that of BHT with FRAP value of （1748.49 ± 3.46） μmol TE/g. The methanol extract from C. kingianus showed the highest antioxidant activity among the four extracts of C. kingianus and A. mengxingensis.