盾叶薯蓣四倍体株系生化指标及有效成分分析

Analysis of Biochemical Indices and Efficient Ingredients in Autotetraploid of Dioscorea zingibiernsis

目的:提高盾叶薯蓣药材的品质。方法:对诱导获得的盾叶薯蓣四倍体株系进行了试管苗生化指标及薯蓣皂苷元含量分析。结果:大部分四倍体株系APX、SOD和POD的活力高于或相近于二倍体株系,可溶性蛋白电泳谱带也表明,四倍体株系与对照株系相比具有一定的差异。经诱导获得的四倍体株系试管苗与对照株系相比薯蓣皂苷元含量有较大差异,大部分四倍体株系薯蓣皂苷元含量都超过了二倍体株系。结论:经诱导获得的四倍体株系在生理生化指标及有效成分含量方面与二倍体表现出明显差异,说明多倍体诱导可以作为盾叶薯蓣优良品种选育的途径。

Objective ： To improve the yield and quality of Dioscrea zingibiernsis. Methods ： The Biochemical indices and the diosgenin＇s content were analysed in the autotetraploid lines. Results： The results showed that the activities of APX, SOD and POD in most of autotetraploid lines were higher than that in diploid line or close to it, there was also difference between autotetraploid lines and control lines in the SDS-PAGE of soluble proteins. The content of diosgenin in most autotetraploid plantlets were higher than that in the control. Conclusion ： There were difference between autotetraploid and control lines in the content of diosgenin and Biochemical indices, therefore,inducing autotetraploid could be an effective way to breeding the superior varieties.