苦参碱对结肠癌SW1116细胞增殖及端粒酶活性的影响

Effects of Matrine on Proliferation and Telomerase Activity of Colon Cancer SW1116 Cells

目的:探讨苦参碱(Matrine,Ma)对人结肠癌SW1116细胞增殖和端粒酶活性的影响。方法:采用MTT法检测细胞增殖抑制率,TRAP-ELISA测端粒酶活性,半定量RT-PCR法检测hTERT mRNA表达。结果:Ma对SW1116细胞具有显著抑制增殖作用,并呈现较明显的剂量及时间依赖性;与正常对照组相比,端粒酶活性显著下降(P<0.05或P<0.01),hTERT mRNA表达明显减少(P<0.05或P<0.01)。结论:Ma能通过下调hTERT表达而有效地抑制SW1116细胞端粒酶活性,从而抑制其增殖。

Objective：To investigate the effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells. Methods ：The proliferation inhibitory rate was evaluated by MTI＇ assay. The telomerase activity was analyzed by TRAP-ELISA, and the expression of hTERT mRNA was determined by semi-quantitative RT-PCR. Results：Matrine displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against SW1116 cells. Compared with control group, the telomerase activity and the expression of hTERT mRNA decreased significantly（P 〈0. 05 or P 〈0. 01 ） in matrine group. Conelusion：Matrine can inhibit the telomerase activity by depressing the expression of hTERT in SW1116 cells and inhibiting cell proliferation.