摘要
目的:观察缩泉丸对肾阳虚多尿模型大鼠肾脏AQP-2 mRNA和AVPR-V2 mRNA表达的影响。方法:用腺嘌呤250mg/kg灌服大鼠4w,造成肾阳虚多尿模型,分别给予缩泉丸或去氨加压素治疗4w,用实时荧光定量PCR法观察缩泉丸对肾阳虚多尿模型大鼠肾脏水通道蛋白AQP-2 mRNA及加压素二型受体(AVPR-V2)mRNA表达的影响。结果:肾阳虚多尿模型组大鼠肾脏AQP-2 mRNA与AVPR-V2mRNA表达比正常对照组明显降低(P<0.01),dDAVP组和缩泉丸高剂量组AQP-2 mRNA表达与AVPR-V2 mRNA表达比模型组明显升高(P<0.05);其余给药各组与模型组相比无显著差异(P>0.05)。结论:缩泉丸能提高肾阳虚多尿模型大鼠肾脏AQP-2 mRNA与AVPR-V2 mRNA表达。
Objective ： To observe the impacts of the formula of Suoquanwan （SQW） on the expression of AQP-2 mRNA and AVPR- V2 mRNA in the kidney of rat polyuria model of Yang-deficieney. Methods ：The model rats were induced by adenine（250 mg/kg） for 4 weeks,then treated respectively with SQW or dDAVP. The expression of AQP-2 mRNA and AVPR-V2 mRNA in kidney of Yang-deficieney model by reahime fluorescence quantitative PCR method were investigated. Results：In model rats, the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney decreased,dDAVP and SQW high dose could increased the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney. The others had no influence on the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney. Conclusion ： SQW can increase the expression of AQP-2 mRNA and AVPR-V2 mRNA in the kidney of rat polyuria model of Yang-deficiency
出处
《中药材》
CAS
CSCD
北大核心
2009年第6期926-928,共3页
Journal of Chinese Medicinal Materials
基金
国家教育部博士点基金(20060572015)
