转化生长因子β1和结缔组织生长因子在大鼠肺纤维化中的表达

A preliminary study of transforming growth factor-β1-mRNA and connective tissue growth factor-mRNA on pulmonary interstitial fibrosis in rats

目的观察转化生长因子β1-mRNA(TGFβ1-mRNA)和结缔组织生长因子-mRNA(CTGF-mRNA)在博莱霉素诱导的大鼠肺间质纤维化中的表达。方法48只雌性Wistar大鼠,随机分为正常组、模型组、每组24只,以气管内注入博莱霉素A2(BLM-A2)建立大鼠肺间质纤维化模型,模型组气管内一次性注入BLM-A2(5mg/kg),正常组气管内注入等量生理盐水。两组大鼠在实验周期第3天、7天、14天和28天用腹主动脉放血法处死进行以下检测:计算肺系数:肺系数=肺湿重(mg)/体重(g),进行HE和VG染色,原位杂交测定大鼠肺组织中TGFβ1-mRNA和CTGF-mRNA的表达情况。结果TGFβ1-mRNA和CTGF-mRNA在博莱霉素诱导的大鼠肺间质纤维化中的表达明显增强。结论TGFβ1-mRNA和CTGF-mRNA的表达增强表明TGFβ1-mRNA和CTGF-mRNA与肺纤维化的发生、发展有较大关联,为本研究后续实验动物模型的药物干预奠定了基础。

Objective To explore the effect of transforming growth factor-β1-mRNA and connective tissue growth factor-mRNA on pulmonary Interstitial fibrosis in rats. Methods 48 female wistar rats were assigned randomly into 2 groups ： model group and normal con- trol group. There were 24 rats in each group. Models were founded by Bleomycin A2. The rats in the model group were filled with bleomycin A2 （5 mg/kg） once in the trachea. The normal control group were filled with equal saline in the trachea. The rats were sacrificed under drugged state at 3, 7, 14, 28 days of feeding, then the pulmonary coefficients were observed in each group. Hematoxylin-eosin staining and van gieson chan biter acid fuchinacid staining were done and the expression of the transforming growth factor β1 -mRNA and connective tissue growth factor-mRNA were detected by the nuclic acid by hybridization in situ technique. Results The expression of TGFβ1-mRNA and CTGF-mRNA in lung tissue increased as fibrosis developed. Conclusion The expression of TGFβ1-mRNA, CTGF, mRNA in lung tissue plays important role in our flowing study.