活体在线法检测肾缺血再灌注过程中抗坏血酸变化研究

In vivo microdialysis coupled with on-line electrochemical detection for continuous monitoring of ascorbic acid in kidney of rabbits following renal ischemia/reperfusion

目的研究缺血再灌注情况下兔肾内抗坏血酸的变化规律。方法实验组及假手术组各8只新西兰兔。实验组动物麻醉后,在肾缺血再灌注过程中利用微透析及电化学分析系统持续检测抗坏血酸的水平。假手术组实验方法同实验组,但不阻断动脉。结果实验组缺血期和再灌注期微透析液内抗坏血酸最高浓度均比缺血前期平均浓度升高,分别升高57.7%、115.9%;再灌注期抗坏血酸的最高浓度比缺血期最高浓度升高37.0%。以上差异均有显著性(P<0.05)。实验组与假手术组各时间段抗坏血酸浓度差异有显著性(P<0.05)。假手术组微透析液内抗坏血酸水平保持相对稳定。结论微透析技术与活体在线电化学检测技术相结合的方法是检测肾抗坏血酸变化的一种很好的手段,抗坏血酸是肾缺血再灌注损伤的一个灵敏指标。抗坏血酸在肾缺血再灌注情况下的变化是多种因素共同作用的结果。

Objective To clarify the change of ascorbic acid in kidney of rabbits following the renal ischemia/ reperfusion. Methods Experiment groups and sham-operation groups both had 8 New Zealand rabbits. Selective electrochemical detection was coupled with in vivo microdialysis for continuous and on-line measurements of ascorbic acid in kidney of experiment rabbits. The procedures of the sham-operated group were the same as those for the experiment group, except that the kidney was not treated into ischemia and reperfusion. Results In the experiment group, microdialysate ascorbic acid from the kidney was increased to 157.7% after ischemia and further to 215.9% after reperfusion, both in relative to the basal level before ischemia （statistical significant, P〈0.05）. The summit concentration of microdialysate ascorbic acid was increased to 137.0% （statistical significant, P 〈 0.05）. While, the microdialysate ascorbic acid from kidney of sham- operated group remained almost unchanged during the experimental time scale. Conclusion Selective electrochemical detection coupled with in vivo microdialysis for on-line measurements is a very effective technique for continuous monitoring.of ascorbic acid in kidney. The change in ascorbic acid could be used as a sensitive indicator for renal ischemia/reperfusion injury and many correlative factors could be responsible for the recorded change in the level of ascorbic acid following the renal ischemia/reperfusion.