CK20和Ki-67在膀胱移行细胞癌中的表达及临床意义

The expression and clinical significance of CK20 and Ki-67 in transitional cell carcinoma of human bladder

目的探讨细胞角质蛋白20(CK20)和细胞核相关抗原(Ki-67)在膀胱移行细胞癌中的表达及临床意义。方法对经临床确诊的膀胱移行细胞癌患者癌组织标本中CK20(66例)和Ki-67(55例)的表达结果进行回顾性分析。结果CK20在膀胱移行细胞癌表达的阳性率为68.2%(45/66)。CK20表达的阳性指数与膀胱移行细胞癌临床分期和细胞分级均呈正相关(P<0.05)。复发膀胱移行细胞癌组CK20阳性表达率92.3%(12/13)高于初发膀胱移行细胞癌组62.3%(33/53)(P<0.05)。Ki-67在膀胱移行细胞癌表达的阳性率为63.6%(35/55)。Ki-67表达的阳性指数与膀胱移行细胞癌细胞分级呈正相关(P<0.05)。复发膀胱移行细胞癌组Ki-67阳性表达率90.9%(10/11)高于初发膀胱移行细胞癌组56.8%(25/44)(P<0.05)。Ki-67和CK20的表达阳性指数呈正相关(r=0.493,P<0.05)。结论研究结果表明CK20和Ki-67的表达与膀胱移行细胞癌的生物学行为相关,因此CK20和Ki-67可作为膀胱移行细胞癌肿瘤病理分级分期以及预后判断的辅助手段。

Objective To study the expression of cytokeratin 20 （CK20） and human nuclear antigen （Ki-67） in bladder transitional cell carcinoma as well as to evaluate their clinical significance. Methods Immunohistochemical method （Envosion） was used to evaluate the expreesion of CK20 in 66 patients and Ki-67 in 55 patients with bladder transitional cell carcinoma. Results The positive expression of CK20 in patients with bladder transitional cell carcinoma was 68.2% （45/ 66）, which was statistically positive correlated with clinical stage and histological grade （P〈0.05）. The positive expression of CK20 in recurrent cases was 92.3% （12/13）, which was significantly higher than that in primary cases （62.3%） （33/53） （P〈 0.05）. The positive expression of Ki-67 in patients with bladder transitional cell carcinoma was 63.6% （35/55）, which was statistically positive correlated with histological grade （P〈0.05）. The positive expression in recurrent cases was 90.9% （10/ 11）, which was significantly higher than that in primary cases （56.8%） （25/44） （P〈0.05）. Conclusion The results suggested that expression of CK20 and Ki-67 in bladder transitional cell carcinoma are related to tumor biological behavior. Therefore, CK20 and Ki-67 can be useful markers of the stage, grade and prognosis of bladder transitional cell carcinoma.