E2F3在前列腺癌中的表达及意义

Expression and significance of E2F3 in prostate cancer

目的探讨E2F3在前列腺癌中的表达及临床意义。方法采用逆转录聚合酶链反应(RT-PCR)和免疫组化技术检测26例前列腺癌、20例良性前列腺增生和10例正常前列腺组织中E2F3mRNA及蛋白的表达。结果E2F3mRNA及蛋白的表达与临床分期有关,晚期(C+D期)E2F3mRNA及蛋白的表达水平明显高于早期(A+B期,P<0.05),低分化癌E2F3mRNA及蛋白的表达明显高于高中分化癌(P<0.05)。前列腺癌中E2F3表达水平明显高于良性前列腺增生(P<0.05)及正常前列腺组织(P<0.05)。结论E2F3表达可能与前列腺癌的恶性程度有关,E2F3在前列腺癌的发生发展中起到促进作用。

Objective To study the expression and clinical significance of E2F3 in prostate cancer （PC）. Methods The expression of E2F3 mRNA and protein in 26 tissues of PC, 20 tissues of benign prostate hyperplasia （BPH） and 10 tissues of normal protate tissue （NP） were detected by reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry. Results The expression of E2F3 mRNA and protein were significantly higher in the tissues from advanced stages （C＋D） than from the early stages （A＋B） （P〈0.05）. The expression level of E2F3 in PC was significantly higher than that in BPH （P〈0.05） and NP （P〈0.05）. Conclusion E2F3 expression might be related to the malignancy of PC, and E2F3 might play a promoting role in the genesis and progression of PC.