巨噬细胞诱发的增殖性玻璃体视网膜病变视网膜内的炎性细胞因子

Inflammatory Cytokines in the Retina of PVR Induced by Macrophages

目的检测增殖性玻璃体视网膜病变（PVR）发生过程中炎性细胞因子在视网膜内的含量变化。方法用同种巨噬细胞诱发免眼PVR，在不同时间点对全层眼组织切片中的TNF－α、IL－1β、IL－8和IL－6进行间接免疫荧光染色，并对其含量变化进行半定量分析。结果此模型1周时，视网膜色素上皮（RPE）胞浆内见极弱荧光绿色（±）；2周时，RPE胞浆内弥散暗荧光绿（＋）；3周RPE胞浆亮绿荧光染色（＋＋），视网膜杆锥层外1／3亦是阳性反应；4周RPE、视杆视锥外2／3呈明亮绿荧光（＋＋＋）。结论四种炎性细胞因子在PVR模型的视网膜组织内含量随自然病程而改变，提示它们在局部病程可能有调控作用。

Objective To examine the inflammatory cytokines in the retina during the development of experimental PVR and to explore their mediation in the wound healing. Method The rabbit eyes after injecting of macrophages were stained with monoclone antibodies of TNF- α, IL-1β, IL-6 and IL - 8 by indirect immunofluorecein and evaluated semi - quantitively. ResultWeak positive staining(±) was showed in RPE cell at first week of the PVR model,stronger(+ ) at second week. There were bright fluorecein green staining(++ )in RPE layer and outer prat of rod and cone layer at third week,diffused and bright positive straining (+++) in the RPE and photoreceptor layer at foutth week. ConclusionThe 4 inf1ammatory cytokines concentrations follow the natural coures of PVR, indicating they may play an irnportant role in mediatlng local wound healing procedure.