PCR技术在链格孢霉鉴定中的应用

Usefulness of PCR Technique in Identification of Alternaria Alternata Allergen Extracts

目的应用PCR技术对链格孢霉进行鉴定,以期获得制备链格孢霉变应原制剂的纯菌种。方法采用空气曝皿收获链格孢霉菌种并进行培养,应用PCR技术对其进行鉴定。结果经空气曝皿、形态学鉴定为链格孢霉菌株的PCR产物经1.5%琼脂糖凝胶电泳分析,紫外灯下鉴定扩增所得条带约580 bp;测序结果经Blast比对,544个碱基中543个能够与链格孢霉26s rDNA的545个碱基比对上,缺失1个,不能识别1个,相符率达99%。结论证实待检真菌为链格孢霉。

Objective To produe the technique. Methods Alternaria technique. Result The fungus allergen extracts with Alternaria alternarta while were identified by PCR alternata was collected from air and cultured under 26℃, identified by PCR that was collected from air and identified as Alternaria alternata by morphology, its 26srDNA was amplified by PCR, resulting a 580 bp band, after sequencing, under microscope there were 543 bases those could be identical and consistent to the Alternaria alternata data from internet based on the result of blast comparison, corresponding rate is 99%. Conclusion We succeeded in collecting and producing A lternaria alternata.