基因重组乳链球菌防龋疫苗主动免疫防龋研究 Ⅱ 皮下注射免疫孕兔的实验研究

Active immunization with genetic recombinant Streptococous lactis vaccine against caries Ⅱ. Local immunization around salivary gland and breasts with recombinant S. lactis HL 107 in pregnant rabbits.

目的：了解基因重组乳链球菌防龋疫苗皮下注射免疫孕兔的抗龋效果。方法：通过酶联免疫吸附检测法分析孕兔免疫前后血清、唾液和乳清中抗变形链球菌表面蛋白抗原的抗体水平。结果：腮腺和乳腺周围区域皮下注射免疫１周～２周后血清ＩｇＧ型特异性抗体、唾液和乳清ＩｇＡ型特异性抗体均有明显升高。结论：基因重组乳链球菌ＨＬ１０７具有变形链球菌表面蛋白抗原的免疫原性。

bjective: To study the anticarious effects of local immunization with recombinant S. lactis HL 107 in pregnant rabbits. Methods: The genetic recombinant S.Lactis  HL107 caries vaccine against caries was injected around salivary gland and breast in pregnant rabbits. The level of antiPAc IgG, IgA in whey, serum and saliva was measured with indirectELISA before and after local subcutaneous injection immunization.Results: Specific antiPAc IgG in serum and antiPAc IgA in saliva and in whey were induced to high level after immunization.Conclusions: The recombinant S. lactis HL 107 possesses immunogenicity of S. mutans surface protein antigen and is able to make the recipient develop specific response against PAc.