放线共生放线杆菌表面相关物质对成纤维细胞L929的细胞毒性作用

The cytotoxicity of surface associated material(SAM) from actinobacillus actinomycetemcomitans(Aa) on fibroblast L929 in vitro

目的：探讨放线共生放线杆菌表面相关物质对成纤维细胞Ｌ９２９的细胞毒性作用。方法：采用ＭＴＴ法、细胞计数法进行细胞毒性检测。结果：放线共生放线杆菌表面相关物质可明显抑制成纤维细胞Ｌ９２９的分裂、增殖，并呈明显的剂量依赖性，１００ｍｇ／Ｌ浓度组与对照组差别显著（Ｐ＜０．０５），但不致细胞死亡。结论：放线共生放线杆菌表面相关物质对Ｌ９２９细胞有明显的毒性作用，其致病机理不同于ＬＰＳ。

Aim:To evaluate the cytotoxicity of SAM from Aa on fibroblast L929. Methods: MTT method and cell count method. Results: SAM from Aa inhibited division and proliferation of L929 cells, at the concentration of 100μg/ml the inhibitary effects was significant compared with that of the control ( P <0.05), no cell lysis was found. Conclusions: The cytotoxicity of SAM from Aa on fibroblast was evident .