放线共生放线杆菌表面相关物质对人牙龈成纤维细胞的增殖抑制作用

The inhibition of surface- associated material(SAM) from Actinobacillus actinomycetemcomitans(Aa) on the growth of human gingival fibroblast

目的：观察放线共生放线杆菌表面相关物质对人牙龈成纤维细胞的增殖抑制作用。方法：采用细胞计数法和图像分析法观察对人牙龈成纤维细胞的生长抑制作用。结果：此物质抑制作用明显，能抑制细胞的分裂、增殖，使细胞、细胞核面积增大，１００ｍｇ／Ｌ对与对照组差别显著（Ｐ＜０．０５），且细胞无死亡现象。结论：此物质可明显抑制人牙龈成纤维细胞的生长、增殖。

Aim:SAM were examined for inhibitory effects on human gingival fibroblast. Methods: Using cell count and image pattern analysis. Results: SAM of Aa could inhibit division and proliferation and lead to increased cytoplasmic and nuclear area. Inhibitory effect was concenrtraition dependent, prounced difference of inhibitory percentage was observed at the concentration of 100 μg/ml compared with that of the control. Conclusions: SAM can inhibiti on the growth of human gingival fibroblast.