荧光定量PCR法与常规法检测解脲支原体和沙眼衣原体的结果对比分析

Fluorescent quantitative polymerase chain reaction compare with Cultivation and Gold--labelled antigen detection of Mycoplasma and Chlaydia trachomatis

目的：调查我院妇科门诊就诊女性生殖道感染解脲支原体（UU）和沙眼衣原体（CT）情况；比较荧光定量PCR法检测UU和CT与UU培养法和CT金标法检测的异同。方法：551例妇女患者取宫颈分泌物，用荧光定量PCR法检测418例分泌物标本的UU—DNA，同时对其中281例标本进行CT—DNA的检测。另133例女性采用支原体培养药敏试剂盒进行UU培养，其中的118例用金标法检测CT，并对结果进行统计、分析。结果：荧光定量PCR法检出UU阳性率54．3％（227／418），培养法检出UU阳性率为54．1％（73／133），经统计差异无显著性；荧光定量PCR法检出CT阳性率9．3％（26／281），金标法检测CT的阳性率1．7％（2／118），经统计差异有显著性。结论：对于UU，荧光定量PCR法与培养法阳性率相当；对于CT，荧光定量PCR法明显优于金标法。

Objective To explore the effect of UU and CT on genitourinary tract of women in our area and study practical value of FQ--PCR in detecting UU and CT. Methods 418 women and 133 women were selected for this study. Cervical secretions of the 418 women were tested for UU DNA by Real time fluorescent quantitative polymerase chain reaetion（FQ--PCR）. 281 eases were tested for CT DNA by FQ--PCR. The cultivation or gold--labelled antigen detection method was used to cultivate UU or test the susceptibility of CT. Results The positive rate of UU（54.3%） by FQ-PCR was simillar as that of the cultivation（54. 1% ）. CT positive rate by FQ--PCR （9.3 %） was higher than that by gold--labelled antigen detection method （1.7 %） . Conclusion It is suggested that FQ--PCR shows practical value in diagnosis and treatment of chlaydia traehomatis infection. Bacteria result cultivation a false conclution in mycoplasma.