18个黑麦草品种(系)的RAPD分析

Genetic diversity of 18 ryegrass varieties determined by RAPD analysis

利用RAPD分子标记技术对18个黑麦草（Loluimsp）品种进行遗传多样性研究。从110个10bp的随机引物中筛选获得26个多态性引物，然后对18份黑麦草种质资源的基因组DNA进行扩增，共获得197条DNA扩增片断带。其中多态性带187条，平均多态检出率为94．9％。18份材料的Jaccard相异系数为0～0．8467，采用UPGMA法聚类分析，结果显示：雅晴、特高、沃土、潘多拉、安格斯、意大利黑麦草、邦德、普通一年生、嘉宝、绿神、普通二倍体、小黑麦为一群，匹克、凯琳特、顶峰、明星、首相为一群，冬牧70为单独一群。

RAPD molecular-marker technique was used to detect the genetic diversity among 18 ryegrass germplasms. Their genomic DNA were tested with RAPD using 26 10-met primers screened from 110 arbitrary 10-mer primers. A total of 197 RAPD bands were obtained, in which 187 were polymorphic. The average percentage of polymorphic bands was 94.9%. The variation range of genetic distances was from 0 to 0. 846 7. By a UPGMA cluster analysis on the RAPD molecular-markers, these ryegrass varieties were divided into three groups： （1） Yatsyn, Tetragold, Pandora, Abundant, Angus, Italian Ryegrass, Barwoltra, Normal Annual Ryegrass, Green Spirit, Normal Diploid and Triticale; （2） Peak, Cailente, Pinnacle, Primer and Bright Star; and （3） Dong Mu 70.