摘要
目的：用高效液相色谱法测定注射用肌苷的含量。方法：采用Kromasil C18柱（5μm 4．6mm×250mm），以甲醇-水（10：90）作流动相，在248nm紫外波长检测。结果：肌苷在4．23-63．48μg／ml范围内有良好的线性关系（r=0．9999）。低、中、高3个浓度的平均回收率（n=2）分别为101．4％、101．8％、101．1％，RSD为0．46％。结论：该法简便、快速、准确，可作为测定注射用肌苷中肌苷的含量。
Objective A high--performance liquid chromatography （HPLC） was established for the determination of inosine contents in injection. Methods Impurity in the inosine injection could be separated on Kromasil C18 colnmn （5μm 4. 6mm × 250mm） using methanol--water （10：90） as mobile phase and UV detector at 248nm. Results The linear range was 4.23-63. 48μg/ml（r=0. 9999）. Average recovery in the lower, moderate and higher inosine concentration groups was respectively 101. 4 %, 101.8 % and 101.1% （n= 2） with RSD of 0.46 %. Conclusion The method is simple, rapid and accurate.
