CACNA2D1基因第14内含子的多态性及其对猪肌肉pH_(45)的影响

Polymorphism of the 14^(th) Intron of Porcine CACNA2D1 Gene and Influence on Muscular pH_(45)

[目的]检测猪CACNA2D1基因第14内含子的多态性,为猪肉质性状的分子标记辅助选择提供条件。[方法]采用PCR-SSCP检测猪CACNA2D1基因第14外显子和内含子在6个猪群中的DNA多态性,并分析不同基因型对猪眼肌和腿臀肌肉pH45的遗传效应。[结果]猪CACNA2D1基因第14内含子有3种基因型,即AA、AG和GG,在其序列(GenBank接受号:FJ156361)的1 145位碱基存在1个多态位点(G→A)。2χ独立性检验结果表明,这3种基因型在各猪群间的分布差异显著(P<0.05)。AA基因型金华×皮特兰F2资源家系猪与AG基因型猪在眼肌和腿臀肌肉pH45值上的差异显著(P<0.05)。[结论]猪CACNA2D1基因第14内含子的多态性影响猪眼肌和腿臀肌pH45值。

[Objective] To detect polymorphism of the14^th intron of porcine CACNA2D1 geue, and thus provide conditions for the marker-assisted selection of pork quality traits. [ Method] The polymorphism of the14^th exon and intron of porcine CACNA2D1 gene was detected in six breeds by PCR-SSCP. The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed. [ Result ] There were three kinds of genotypes, namely, AA, A G and GG, in the 14^th intron of porcine CA CN42D1 gene. The polymorphie locus （G → A） was at the 1 145 nt of the sequence （GeuBank Accepted No. FJ156361 ）. The results of x^2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds （P 〈 0.05）. The pH45 values of eye muscle and ham joint muscle were significantly different between genotype AA and genotype AG of F2 generation from the Jianhua × Pietrain resource family （P 〈0. 05）. [ Conclusion] The polymorphism of the4^th intron of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.