高原农作物豌豆抗氧化和脂质过氧化研究

Research of Antioxidants and Lipid Peroxidation from Pea Crops of Plateau

[目的]探讨高原农作物豌豆提取液抗氧化和脂质过氧化的作用。[方法]对豌豆进行SOD酶液的提取,测定蛋白浓度、酶活力、抗氧化活性、抑制小鼠离体组织过氧化能力、体外组织脂质过氧化能力。[结果]豌豆粗酶液对DPPH.的EC50为55.16 mg/L,清除率低于同浓度的Vc和茶多酚、柠檬酸,Vc与粗酶液均有协同效应,相对而言,Vc的增效优于柠檬酸。豌豆SOD酶液对DPPH.的EC50为11.1 mg/L,优于茶多酚,与维生素C非常接近。豌豆SOD酶液对小鼠离体肝、肾和心组织中的MDA生成均有抑制作用,其中对离体肝组织中MDA生成的抑制效果最明显,浓度为0.25 mg/m l时,抑制率为78.3%,此后再增大浓度,抑制率的增加不多,心和肾效果次之。[结论]豌豆SOD粗酶液与豌豆SOD酶液都有一定清除DPPH.的能力,豌豆SOD酶液有抑制脂质过氧化能力。

[ Objective ] The effect of the antioxidants and lipid peroxidation from pea crops of plateau was studied. [ Method ] Pea SOD enzyme liquid was extracted. And protein concentration, enzyme activity, antioxidant activity,inhibitory of tissue departing from the body peroxide activity and that of the lipid peroxidation of tissues were measured. [ Results] According to the 50% scavenging concentration EC50 on DPPH · , EC50 of the crude pea crops of plateau on DPPH· was 55.16 mg/L. The scavenging rate of the crude naked barley was lower than ascorbic acid and tea polyphenol of the same concentration. Meanwhile, when citric acid and ascorbic acid were used together with pea crops of plateau, ascorbic acid showed stronger synergistic antioxidant activities with pea crops of plateau. ECho of the pea crops of plateau on DPPH· was l 1.1 mg/L, which was lower than tea polyphenol and was close to ascorbic acid. And the pea crops of plateau had obvious effect on inhibiting tissue homogenate MDA. By comparison of the inhibitory rate of tissue homogenate MDA of heart, liver and kidney, it was found that its effect on liver was the best with inhibitory rate 78.3% when the pea crops of plateau concentration was 0.25 mg/ml. [ Conclusion] The crude and the pea crops of plateau all had effects on scavenging DPPH· free radical and inhibiting lipid peroxidation.