摘要
2008年3月自澳大利亚进口的豌豆种苗病变部位分离到致病性菌株,该菌株能引起豌豆茎部、卷须萎蔫腐烂,叶片褪绿等症状。针刺接种豌豆,发生的病变状态与自然发病的症状相同,且在接种发病植株中又分离到形态上一致的病原菌,得到了柯赫法则的验证。经形态特征、培养性状、生理生化及16S rDNA序列分析,鉴定其为桃色欧文氏菌(Erwinia persicina)。这是我国首次从进境豌豆中检出该病害。
Three isolates were isolated from diseased seedling stems of pea imported from Australia in March 2008. Inoculation on pea seedlings with these isolates caused the same symptoms as naturally infected plants, while symptoms did not occur on control plants （inoculated with sterile water）. The bacteria sharing the characteristics of the inoculated isolates were recovered from symptomatic plants, hence fulfilling Koch＇s postulates. All three isolates were identified as Erwinia persicina by pathogenicity, staining reactions, morphological characteristics, culture patterns, physiological and biochemical reactions, and 16S rDNA gene sequences.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第3期238-242,共5页
Acta Phytopathologica Sinica
基金
“十一五”国家科技支撑项目(2006BAD08A16)
关键词
豌豆
细菌性病害
病原鉴定
桃色欧文氏菌
pea
bacterial disease
pathogen identification
Erwinia persicina
