不同民族中PreS_2Ag与PreS_2Ab水平测定及意义

The Significance of Detection of the Level of PreS_2Ag and PreS_2Ab in Diffirent Ntionalities

目的测定新疆地区汉族、维吾尔族(维族)、哈萨克族(哈族)、回族及蒙古族中HBV感染者PreS2Ag与PreS2Ab水平,观察其与HBV DNA载量之间的相关性。方法使用荧光定量PCR法进行HBV DNA载量测定,应用ELISA法确定PreS2Ag和PreS2Ab含量。结果测定了317例不同民族的HBV感染者血清,177例HBV DNA+组中,PreS2Ag阳性157例,PreS2Ab阳性10例,阳性率分别为88.70%和5.65%;在140例-组中,PreS2Ag阳性112例,PreS2Ab阳性14例,阳性率为80.00%、10.00%。结论在新疆各民族之间,HBV感染者血清HBV DNA+和HBV DNA-组中的PreS2Ag的阳性率,经统计学处理后无显著性差异(χ2<3.84,P>0.05)。在HBV DNA-组中,仅有汉族与哈族,维族与哈族中PreS2Ab的阳性率的比较有显著性差异(χ2=13.78和9.00,P<0.005)。

Objective To analyse the level of PreS2Ag and PreS2Ab of the HBV-infected persons in Han,Uygur,Hazakh,Muslim,Mongolia people who live in Xinjiang area,and observe the relationship between the Ag,Ab and HBV DNA. Methods detecting the HBV DNA with automatic fluorescent quantitation PCR,detecting the level of PreS2Ab and PreS2Ag with ELISA. Results Detecting the 317 HBV-infected persons,among the 177 HBV DNA＋ patients, 157 are positive of PreS2Ag, 10 were positive of PreS2Ab,the rates of positive are 88.70% and 5.65%. Among the 140 HBV DNA- patients, 112 were positive of PreS2Ag, 14 are positive of PreS2Ab,the rates of positive are 80.00% and 10.00%. Conclusion There is no significant difference about the positive rates of PreS2Ab and PreS2Ag of the HBV-infected persons in the nationalities of Xinjiang （ X^2 〈 3.84, P 〉 0.05 ）. Among the 140 HBV DNA- patients, there are significant difference of the positive rate of PreS2Ab between Han and Hazakh, Uygnr and Hazakh（ X^2= 13.78 and 9.00, P 〈 0.005 ）.